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linear rnas  (New England Biolabs)


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    Structured Review

    New England Biolabs linear rnas
    Linear Rnas, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 2461 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/linear+rnas/T4+RNA+Ligase+2/pm39965568-572-14-23
    Average 98 stars, based on 2461 article reviews
    linear rnas - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Purification:

    Article Title: Rolling circle reverse transcription enables high fidelity nanopore sequencing of small RNA.
    Article Snippet: .. Linear RNAs were then degraded by adding the following mixture of enzymes 2uL XRN-1 (NEB M0338), 1uL 5’deadenylase (NEB M0331), RNase R (purified in house), ATP and Poly(A) Polymerase (NEB M0276L). .. The reaction mixture was then diluted to 80 μL with 2x Induro RT buffer (1x final concentration), 1 μM primer (S1 Table, oligo 2), 1 mM dNTPs and 100 ng of Induro RT (NEB M0681).

    Article Title: Rolling circle reverse transcription enables high fidelity nanopore sequencing of small RNA
    Article Snippet: .. Linear RNAs were then degraded by adding the following mixture of enzymes 2uL XRN-1 (NEB M0338), 1uL 5’deadenylase (NEB M0331), RNase R (purified in house), ATP and Poly(A) Polymerase (NEB M0276L). .. The reaction mixture was then diluted to 80 μL with 2x Induro RT buffer (1x final concentration), 1 μM primer ( , oligo 2), 1 mM dNTPs and 100 ng of Induro RT (NEB M0681).

    Article Title: Degradation of circular RNA by the ribonuclease DIS3.
    Article Snippet: Short article

    Incubation:

    Article Title: Coordinated circRNA Biogenesis and Function with NF90/NF110 in Viral Infection.
    Article Snippet: .. 50 mg linear RNAs were incubated with T4 RNA ligase 1 (NEB) in 500 mL reaction for overnight at 16 C according to the manufacturer’s protocol. ..

    Article Title: Structure and Degradation of Circular RNAs Regulate PKR Activation in Innate Immunity.
    Article Snippet: .. For in vitro circularization, 50 mg linear RNAs were incubated with T4 RNA ligase 1 (NEB) in 500 mL reaction for overnight at 16 C according to the manufacturer’s protocol. ..

    Article Title: Linking circular intronic RNA degradation and function in transcription by RNase H1.
    Article Snippet: School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China; State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China; CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China; Current address: Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02115, USA


    Article Title: Degradation of circular RNA by the ribonuclease DIS3.
    Article Snippet: Short article

    In Vitro:

    Article Title: Structure and Degradation of Circular RNAs Regulate PKR Activation in Innate Immunity.
    Article Snippet: .. For in vitro circularization, 50 mg linear RNAs were incubated with T4 RNA ligase 1 (NEB) in 500 mL reaction for overnight at 16 C according to the manufacturer’s protocol. ..

    Article Title: Linking circular intronic RNA degradation and function in transcription by RNase H1.
    Article Snippet: School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China; State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China; CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China; Current address: Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA 02115, USA

    Article Title: Degradation of circular RNA by the ribonuclease DIS3.
    Article Snippet: Short article



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